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Revision as of 04:19, 14 September 2015
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__NOTOC__
__NOTOC__
<partinfo>BBa_K1595030 short</partinfo>
<partinfo>BBa_K1595030 short</partinfo>
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Use in conjunction with strain-expressing T7 polymerase.
Use in conjunction with strain-expressing T7 polymerase.
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cel5a
is a stable, detectable protein that can be used to determine expression of our plasmid.
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Cel5a
is a stable, detectable protein that can be used to determine expression of our plasmid.
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EDA had an internal PstI cutsite that was removed by altering the DNA sequence.
===Source===
===Source===
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===References===
===References===
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EDA: NCBI accession number P0A955
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Cel5a: Pereira et al. <i>Biochemical characterization and crystal structure of endoglucanase Cel5A from the hyperthermophilic Thermotoga maritima</i>. Journal of Structural Biology. Vol 172(3). Dec 2010. 372-379.