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__NOTOC__
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<partinfo>BBa_K1298001 short</partinfo>
<partinfo>BBa_K1298001 short</partinfo>
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===Design Notes===
===Design Notes===
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NA
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While considering the design of the part, a change to the DNA sequence was required in order to avoid having the protein code cut at unwanted points (illegal cut sites) when doing a restriction digest. The DNA optimization was achieved. It should be noted that, in order to get the chitinase into a pSB1C3 backbone, the part BBa_J04450 was used. The Red Fluorescent Protein (RFP) was removed and the PgeChia 1-1 protein code was inserted. It was done in this way in order to check that the E. coli bacteria being use had obtained the chitinase (Red colonies would not have picked up the PgeChia 1-1, but the white ones would have).
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===Source===
===Source===
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Phytochemistry paper of Dr. Kolosova (1), NCBI (code; http://www.ncbi.nlm.nih.gov/nuccore/HM219843), Bio Basic Inc. (DNA optimization)
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Cloned from Lodgepole pine - check notebook
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===References===
===References===
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1. Kolosova, N. “Cloning and characterization of chitinases from interior spruce and lodgepole pine.” 1 Oct. 2013. Feb. 2014 <http://www.ncbi.nlm.nih.gov/pubmed/24564978>