The Penridge Suite, 470 Bowes Road, London N11 1NL: Wednesday, 17 October 2012
This discussion forum focuses on the technique of immunohistochemistry, from DAB to double staining. The invited experts will be give short overview presentations on their sub-topics, with the majority of the day dedicated to informal question and answer troubleshooting sessions.
Meeting Chair: Dr Will Howat, Head of Histopathology/ISH at Cambridge Research Institute, Cancer Research UK
This event has CPD accreditation
On registration please submit your questions to the panel that will be asked by the chair on the day of the event
9:00 – 9:30 Registration
9:30 – 9:35 Introduction by Meeting Coordinator: Dr Astrid Englezou , Euroscicon, London, UK
9:35 – 9:40 Introduction by the Chair: Dr Will Howat, Head of Histopathology/ISH at Cambridge Research Institute, Cancer Research UK
9:35– 9:55 Title to be confirmed
Dr Will Howat, Head of Histopathology/ISH at Cambridge Research Institute, Cancer Research UK
9:55 – 10:15 Immunohistochemistry for Glycol Methacrylate embedded samples
Dr Susan J Wilson PhD, Head of the Histochemistry Research Unit, Southampton General Hospital
Glycol methacrylate (GMA) was introduced in the 1960s and is a hydrophilic acrylic resin. By using a modified processing and embedding procedure it can be used for immunohistochemical studies. This offers several advantages over traditional techniques including good antigen preservation, excellent morphology and antigen localisation, no need for antigen retrieval, cutting of numerous serial sections is possible and co-localisation studies. We use an avidin-biotin peroxidase detection system and have applied this methodology to a wide range of samples including lung, gut, kidney, skin, conjunctiva, tissue culture monolayers and dispersed cells. Details of the technique including fixation, processing, sectioning and the immunohistochemical protocol will be discussed.
10:15 – 10:35 Talk to be confirmed
10:35 – 10:55 Title to be confirmed
Urban Ryberg, The Human Protein Atlas project, Uppsala, Sweden
10:55 – 11:25 Mid-morning Break
Please try to visit all the exhibition stands during your day at this event. Not only do our sponsors enable Euroscicon to keep the registration fees competitive, but they are also here specifically to talk to you
11:25 – 11:45 Making the most of tissue microarrays
Dr Anthony Warford, Warford Technology Ltd, UK
Tissue microarrays (TMAs) have revolutionised the way in which research is done in cellular pathology. In essence, they miniaturise the amount of tissue and time required to generate statistically meaningful results. To achieve this, TMAs must be prepared carefully. First and foremost, tissue cores from each donor sample must be representative of the histology or pathology. To achieve this guided tissue selection must be used and consideration given to the diameter and number of cores removed from each donor sample. Led by the research question to be answered the number of donor samples in each TMA should also be carefully considered. Properly made hundreds of sections can be cut from a TMA block and these should be protected from antigen degradation before staining. Lastly, once staining has been completed, analysis should be undertaken in a consistent manner to maximise the return on the investment in TMA preparation.
11:45 – 12:05 Talk to be confirmed
12:05 – 12:40 Working Lunch
Please collect your lunch and take it to your discussion table (Session 1)
This is also a good time to fill out your feedback forms
12:40 – 14:10 Discussion Group Sessions 1 – 3
Round table discussion groups (20 minutes each) will be held throughout the afternoon
Delegates will rotate so that they may participate in all the discussion tables
All delegates will also be allocated a session for visiting the exhibition stands
Where appropriate delegates will be able to bring their samples to the discussions
See end of agenda for description of discussion tables
14:10 – 14:30 Tissue Cross-reactivity Studies: current considerations
Joanne Mitchell, Huntingdon Life Sciences, Huntingdon, UK
14:30 – 15:35 Discussion Group Sessions 4 – 6
15:35 – 16:20 Question and Answer Session
This session will include summing up of the discussion tables and questions submitted both prior to the meeting and throughout the day
16:20 – 16:30 Chairman’s Summing Up and Feedback Prize Draw
Discussion tables
Table A: Will Howat
Table B: Susan J Wilson
Table C: Urban Ryberg
Table D: Anthony Warford,
Table E: TBC
Table F: TBC
Keywords: Tissue Microarrays, immunohistochemistry, fixation, imaging, quantitation,glycol methacrylate resin, immunohistochemistry, fixation
About the Chair
Will Howat graduated with a BSC (Hons) in Immunology & Pharmacology from the University of Strathclyde, before gaining a PhD in Pathology from the University of Southampton. After two post-doctoral positions in Southampton, he moved to the Wellcome Trust Sanger Institute in Cambridge as the leader of Research & Development for the Immunohistochemistry group of the Atlas of Protein Expression project. He is now with Cancer Research UK as the head the Histopathology/ISH facility at the Cambridge Research Institute.
About the Speakers
Anthony (Tony) Warford expertise is in molecular histopathology. He has set up and managed laboratories in the UK health service, academic institutions, biotechnology and Pharmaceutical companies. Technology developments he has spearheaded include the introduction of diagnostic immunohistochemical methods, validation of antibodies for use as biomarkers, production of probes and methods for in situ hybridisation and supervision and interpretation of GLP tissue based safety studies of potential therapeutic antibodies. Concurrently he has championed quality assurance programmes in histopathology and automation of immunohistochemistry coupled with image capture and analysis. He has also run laboratory safety and human bio-banking programmes. He has published in these fields and shared experience with fellow scientists by organising wet workshops, chairing symposia and lecturing in many countries.
Susan Wilson initially trained as a NHS Biomedical Scientist in histopathology. After gaining her Fellowship of the Institute of Biomedical Sciences in Cellular Pathology, she took a position at the University of Southampton. Susan was a founding member of Professor Holgate’s asthma bronchoscopy research programme, providing histopathology support which furthered the understanding of the pathobiology of asthma and during this time she studied for a PhD in Mucosal Inflammation. Susan has over 25 years experience in histopathology and now heads the Histochemistry Research Unit (HRU) within the Faculty of Medicine. The HRU is a core facility offering a full histopathology support service. The Unit is internationally recognised for its histopathology expertise, being particularly known for the use of glycol methacrylate (GMA) resin embedding for immunohistochemistry procedures.
Joanne Mitchell holds a B.Sc. Hons degree in Applied Biology with European Studies and a Masters degree in Biomedical Science. She has over 13 years experience in immunohistochemistry (IHC) and histopathology gained from five years working at Pharmagene in ‘Molecular Imaging’ pre-clinical research projects, three years managing operations in ‘The Atlas Project’ IHC laboratory at The Sanger Institute and two years setting-up and running a Histopathology/IHC Core Facility for Cancer Research UK. Jo joined HLS in 2008 as Head of Special Pathology Services. She is responsibile for IHC, tissue cross-reactivity, electron microscopy and micro-autoradiography studies, working to GLP.
Media Partners
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This meeting was organised by Euroscicon (www.euroscicon.com), a team of dedicated professionals working for the continuous improvement of technical knowledge transfer to all scientists. Euroscicon believe that they can make a positive difference to the quality of science by providing cutting edge information on new technological advancements to the scientific community. This is provided via our exceptional services to individual scientists, research institutions and industry.
Registration Web Site: www.regonline.co.uk/IHCforum2012
Post expires at 2:27pm on Thursday October 18th, 2012
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