Protocol

Recovery of Replication-Competent and G-Gene-Deleted Rabies Viruses from cDNA

  1. Karl-Klaus Conzelmann
Adapted from Imaging in Neuroscience: A Laboratory Manual (ed. Helmchen and Konnerth). CSHL Press, Cold Spring Harbor, NY, USA, 2011.

Abstract

This protocol describes the recovery of replication-competent rabies viruses (RV) such as SAD L16 or SAD L16-eGFP. It is suggested that at least three parallel transfection experiments are performed to increase the success rate (in three 3.5-cm2 dishes or in three wells in a six-well plate). The entire protocol takes 10 d, and successful rescue can be obtained after 6 d. An additional procedure for the recovery of G-gene-deleted viruses is also included. Please note that appropriate biosafety measures are needed.

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  1. doi:10.1101/pdb.prot089409 Cold Spring Harb Protoc 2015: pdb.prot089409- © 2015 Cold Spring Harbor Laboratory Press
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